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Auntie Mo is at it again. In a new clip from her comedy set, as the opening act on Katt Williams’ comedy tour, Mo’Nique told the audience that Oprah Winfrey and Tyler Perry are “coon motherf****rs.”

The comedian even got the audience involved leading the crowd in a chant saying, “F**k you, Oprah Winfrey. F**k you, Tyler Perry.”

The one-sided feud goes back years from when the comedian actor accused the billionaire media moguls and film producers of blacklisting her and intentionally stalling her career, after she refused to do press for the movie “Precious” for free.

In a nearly three-hour interview on “Club Shay-Shay,” Mo’Nique again claimed to have audio of Perry admitting to blackballing her by spreading rumors about her being difficult to work with. In the recording, he allegedly admits that it was the wrong thing to do.

“I believe you are brilliantly talented and you should have a lot more happening since you won that award,” Perry said, presumably referencing Mo’Nique’s 2010 Oscar for Best Supporting Actress in “Precious.”

He added, “I thought for sure if you had campaigned…and played by their rules, I believe that in the next deal, you would’ve gotten more money, millions of dollars, and your career in the field would’ve been much different. I believe that much in your talent.”

It would seem that the comedian has room to sue Perry, but why won’t she? And why won’t he just settle with her and make her go away for good. As they say, “There are questions that need answers.”

During her performance, Mo’Nique asked an audience member if he was a fan of Winfrey, the man replied affirmatively, and Mo replied, “OK, well I’m not apologizing for a motherf–king thing I say.”

She even doubled down and alleged that Winfrey and her lifelong best friend Gayle King have a more than platonic relationship, according to a transcript published by Page Six. “Every time you see f–king Oprah, you see f–king Gayle,” she said. “Best friends is what they say. I’ve never seen best friends like that … Who is loving Oprah Winfrey and Gayle King? Each other.”

Although Mo may have been able to get some audience members to join her in her rant, the consensus on social media is that the 56-year-old comedian needs to move on.

“I wish Monique the best. You could not pay me to spend my $ currently to go see her bitch about Tyler and Monique. Precious came out in 2009. Somebody please tell her to get back to the jokes. She said it all in the Shannon interview.”